脫氧核糖核酸酶Ⅱ 來源于豬脾(純化,溶液), ≥12,000 units/mg protein

??【編號】：128605

??【產(chǎn)品名稱】：脫氧核糖核酸酶Ⅱ 來源于豬脾(純化,溶液), ≥12,000 units/mg protein

??【規(guī)格】：1KU

??【用途】：

　　脫氧核糖核酸酶Ⅱ 來源于豬脾(純化,溶液), ≥12,000 units/mg protein

　　Product Name： Deoxyribonuclease II from porcine spleen(Purified,Solution)

　　CAS號：9025-64-3

　　介紹： Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

　　用途： DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.

　　貯存：儲存溫度 -20°C

　　生化和生理學(xué)機理： The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

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脫氧核糖核酸酶Ⅱ 來源于豬脾(純化,溶液), ≥12,000 units/mg protein

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脫氧核糖核酸酶Ⅱ 來源于豬脾(純化,溶液), ≥12,000 units/mg protein

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