脫氧核糖核酸酶Ⅱ 來源于豬脾, ≥800 units/mg dry weight
| ??【編號】：128600 ??【產品名稱】：脫氧核糖核酸酶Ⅱ 來源于豬脾, ≥800 units/mg dry weight ??【規格】：80KU ??【用途】：   脫氧核糖核酸酶Ⅱ 來源于豬脾, ≥800 units/mg dry weight
Product Name： Deoxyribonuclease II from porcine spleen
CAS號：9025-64-3
介紹： Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
用途： DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
貯存： 儲存溫度2-8℃
生化和生理學機理： The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.
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