293T人胚腎細(xì)胞
描述：293細(xì)胞株插入SV40 T-抗原的溫度敏感基因后產(chǎn)生的高轉(zhuǎn)染效率的衍生株稱為293T。
動物種別：人
性別：***
組織來源：腎
形態(tài)：上皮細(xì)胞
培養(yǎng)基和添加劑：DMEM培養(yǎng)基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。
供應(yīng)限制：A。僅供研究之用。
REFERENCE：Sena-Esteves M , et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361 Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999 Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001 Pear WS , et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960
中文名稱：人胚腎細(xì)胞
